| Source | Human 293 Cells | M.W. | 45kDa | CAS No. | |
|---|---|---|---|---|---|
| Structural Info | |||||
| Formulation | Lyophilized in sterile filtered solution of PBS. | ||||
| Reconstitution | Before reconstitution, a brief spin is recommend to drive down any material dislodged from the bottom of the tube. The lyophilized protein should be reconstituted in sterile H2O to a desired concentration. | ||||
| Stability | The lyophilized protein is stable for at least one year if stored at -80°C. Reconstituted protein is stable for at least four weeks at 4 °C, but should be stored in aliquots at -80 °C for longer term. Avoid repeated freeze and thaw. | ||||
| Purity | Greater than 95% as determined by SDS-PAGE analysis and visualized by silver staining. | ||||
| Biological Activity | The activity was determined by using a HUVEC cells. The ED 50 ranges from 2- 10 ng/ml. Activity in other assays should be determined by each individual setting. | ||||
| Country of Origin | USA | ||||
VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, it plays a prominent role in normal and pathological angiogenesis. StemRD’s VEGF-165 is made in human 293 cells as a 45kDa glycoprotein consisting of two 165 amino acid polypeptide chains.It is purified by aseries of chromatography.
| Catalog No. | Size |
| VEGF-010 | 10µg |
| VEGF-050 | 50µg |
| VEGF-250 | 250 µg |
| VEGF-1000 | 1000µg |
StemRD 是美国一家聚焦于尖端干细胞研究的公司,拥有蛋白表达和纯化方面的专利技术,使StemRD能够保证高效的生产那些用传统方法很难得到的重要干细胞因子。StemRD 拥有被美国FDA认证的可用于GMP生产治疗型多肽的设施。StemRD的实验室也拥有包括组织培养、细胞处理、超离心、超过滤、FPLC、HPLC和LC/MS等一系列先进设备。



