Source | E. coli | M.W. | 25 kDa | CAS No. | |
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Structural Info | |||||
Formulation | Lyophilized from sterile filtered PBS solution, carrier-free. | ||||
Reconstitution | Before reconstitution, we recommend a brief spin to drivedown any material dislodged from the bottom of the tube. It is recommended to reconstitute the lyophilized product inacid solution such as 1mM HCl. Furtherdilutions should be made in sterile buffer or medium containing carrierproteins, such as albumin or serum. | ||||
Stability | The lyophilized protein is stable for at least 1 yearif stored at -80 °C. Reconstituted protein is stable for at least 2 week at 4 ° C, but shouldbe stored in aliquots at -80 °C for longer term. Avoidrepeated freeze/thaw. | ||||
Purity | Greater than 95% by SDS-PAGE analysis | ||||
Biological Activity | The activity was determined by proliferation of the NIH3T3 cells.The EC50 ranges from 1 – 5 ng/ml. | ||||
Country of Origin | USA |
Platelet-Derived Growth Factor (PDGF) is a mitogen for manycell types, such as fibroblast, smooth muscle cells and endothelial cells. StemRD produces PDGF-bb in Escherichia coli as a disulfide bond-linked homodimer with amolecular mass of 25 kDa. Thisproduct is suitable for tissue culture uses.
StemRD 是美国一家聚焦于尖端干细胞研究的公司,拥有蛋白表达和纯化方面的专利技术,使StemRD能够保证高效的生产那些用传统方法很难得到的重要干细胞因子。StemRD 拥有被美国FDA认证的可用于GMP生产治疗型多肽的设施。StemRD的实验室也拥有包括组织培养、细胞处理、超离心、超过滤、FPLC、HPLC和LC/MS等一系列先进设备。