Stemrd/WNT-5a, mouse/25 µg/W5A-M-025,Stemrd,,Stemrd,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Stemrd/WNT-5a, mouse/25 µg/W5A-M-025

产品编号：W5A-M-025

市场价：¥22900.00

场地：美国(厂家直采)

产品分类：抗体类>二抗>抗大鼠>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$1145.00

品牌： Stemrd

公司：Stemrd

公司分类：

商品介绍

Source		M.W.	~45 kDa	CAS No.
Structural Info
Formulation	Lyophilized in sterile filtered solution of PBS with 2% CHAPS
Reconstitution	Before reconstitution, werecommend a brief spin to drive down any material dislodged from the bottom ofthe tube. The lyophilized protein shouldbe reconstituted in sterile H2O to a concentration of 100 ng/uL. Because of the hydrophobic nature of thisprotein, further dilutions should be made in buffer or medium containingcarrier proteins, such as albumin or serum.
Stability	The lyophilized protein isstable for at least 1 year if stored at -80 °C. Reconstituted protein is stable for at least1 month at 4 °C, but should be stored in aliquots at -80 °C forlonger term. Avoidrepeated freeze and thaw.
Purity	Greater than 85% as determined by SDS-PAGE and HPLC analysis
Biological Activity	The activity was determined by using a TCF reporter gene assay in 293 cells co-transfected with Frizzled-4 and LRP-5.WNT-5a activates (instead of inhibits) the TCF reporter gene in this assay.
Country of Origin	USA

WNT-5a belongs to the class of WNT proteins thatactivate the “non-canonical” pathway. Thepredicted size of mouse WNT-5a is a monomeric protein containing 356 amino acidresidues. Due to glycosylation, itmigrates at an apparent molecular weight of ~45 kDa by SDS-PAGE analysis undernon-reducing conditions. StemRD’sproduct is expressed from a mouse cell line, and purified with a proprietaryprocess that is distinct from the published method.

Product Sheet

Ishida-Takagishi M, Enomoto A, etal., The Dishevelled-associating protein Daple controls the non-canonicalWnt/Rac pathway and cell motility. NatureCommunication. 2012May 29;3:859 http://www.ncbi.nlm.nih.gov/pubmed/22643886Nakamuta S, Funahashi Y, Namba T etal., Local application of neurotrophins specifies axons throughinositol 1,4,5-trisphosphate, calcium, and Ca2+/calmodulin-dependentprotein kinases. ScienceSignal. 2011 Nov 15;4(199):ra76.http://www.ncbi.nlm.nih.gov/pubmed/22087032

品牌介绍

StemRD 是美国一家聚焦于尖端干细胞研究的公司，拥有蛋白表达和纯化方面的专利技术，使StemRD能够保证高效的生产那些用传统方法很难得到的重要干细胞因子。StemRD 拥有被美国FDA认证的可用于GMP生产治疗型多肽的设施。StemRD的实验室也拥有包括组织培养、细胞处理、超离心、超过滤、FPLC、HPLC和LC/MS等一系列先进设备。

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Small Molecules Growth Differentiation Bioactive Peptides Pathway Regulators Media for Stem Cells

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