| Source | M.W. | ~40 kDa | CAS No. | ||
|---|---|---|---|---|---|
| Structural Info | |||||
| Formulation | Lyophilized in sterile filtered solution of PBS with 1% CHAPS | ||||
| Reconstitution | Before reconstitution, werecommend a brief spin to drive down any material dislodged from the bottom ofthe tube. The lyophilized protein shouldbe reconstituted in sterile H2O to a concentration of 100 ng/uL. Because of the hydrophobic nature of thisprotein, further dilutions should be made in buffer or medium containing carrierproteins, such as albumin or serum. | ||||
| Stability | The lyophilized protein isstable for at least 1 year if stored at -80 °C. Reconstituted protein is stable for at least 1month at 4 °C, but should be stored in aliquots at -80°C for longerterm. Avoid repeated freeze andthaw. | ||||
| Purity | Greater than 90% as determined by SDS-PAGE analysis | ||||
| Biological Activity | The activity was determined by using a TCF reporter gene assay in NIH3T3 cells.The EC50 ranges from 50 - 150 ng/ml. | ||||
| Country of Origin | USA | ||||
WNT-3a is a member of the WNT family ofsignaling proteins that play a key role in embryonic development and theintegrity of adult tissues. WNT-3a is aprototypic canonical WNT that signals through the b-catenin pathway. The predicted size of mouse WNT-3a is amonomeric protein containing 333 amino acid residues. Due to glycosylation, it migrates at anapparent molecular weight of 40 kDa by SDS-PAGE analysis under non-reducingconditions. StemRD’s product isexpressed from a mouse cell line overexpressing mouse WNT-3a. Purification is performed with a proprietaryprocess that is distinct from the published method.
StemRD 是美国一家聚焦于尖端干细胞研究的公司，拥有蛋白表达和纯化方面的专利技术，使StemRD能够保证高效的生产那些用传统方法很难得到的重要干细胞因子。StemRD 拥有被美国FDA认证的可用于GMP生产治疗型多肽的设施。StemRD的实验室也拥有包括组织培养、细胞处理、超离心、超过滤、FPLC、HPLC和LC/MS等一系列先进设备。
暂无问答
本网站将在规定时间内给予删除等相关处理。
