产品编号：W5A-H-025
市 场 价：¥27900.00
场 地：美国(厂家直采)
联系QQ：1570468124
电话号码：4000-520-616
邮 箱：
info@ebiomall.com
商品介绍
| Source | M.W. | ~45 kDa | CAS No. | ||
|---|---|---|---|---|---|
| Structural Info | |||||
| Formulation | Lyophilized in sterile filtered solution of PBS with 1% CHAPS | ||||
| Reconstitution | Before reconstitution, werecommend a brief spin to drive down any material dislodged from the bottom ofthe tube. The lyophilized protein shouldbe reconstituted in sterile H2O to a concentration of 100 ng/uL. Because of the hydrophobic nature of thisprotein, further dilutions should be made in buffer or medium containingcarrier proteins, such as albumin or serum. | ||||
| Stability | The lyophilized protein isstable for at least 6 months if stored at -80 °C. Reconstituted protein is stable for at least 2weeks at 4 °C, but should be stored in aliquots at -80 °C forlonger term. Avoidrepeated freeze/thaw. | ||||
| Purity | Greater than 85% as determined by SDS-PAGE and HPLC analysis | ||||
| Biological Activity | The activity was determined by using a TCF reporter gene assay in 293 cells transfected with Frizzled-4 and LRP-5.WNT-5a activates (instead of inhibits) the TCF reporter gene in this assay. | ||||
| Country of Origin | USA | ||||
WNT-5a belongs to the class of WNT proteins thatactivate the “non-canonical” pathway. Thepredicted size of human WNT-5a is a monomeric protein containing 357 amino acidresidues. Due to glycosylation, itmigrates at an apparent molecular weight of ~45 kDa on SDS-PAGE undernon-reducing conditions. StemRD’sproduct is expressed from a human cell line in animal-free medium, and purifiedwith a proprietary process that is distinct from the published method.
Product Sheet
Yamada A, Iwata T, Yamato M et al., Diverse functionsof secreted frizzled-related proteins in the osteoblastogenesis of human multipotentmesenchymal stromalcells. Biomaterials. 2013 Apr;34(13):3270-8.http://www.ncbi.nlm.nih.gov/pubmed/23384792
品牌介绍
StemRD 是美国一家聚焦于尖端干细胞研究的公司，拥有蛋白表达和纯化方面的专利技术，使StemRD能够保证高效的生产那些用传统方法很难得到的重要干细胞因子。StemRD 拥有被美国FDA认证的可用于GMP生产治疗型多肽的设施。StemRD的实验室也拥有包括组织培养、细胞处理、超离心、超过滤、FPLC、HPLC和LC/MS等一系列先进设备。
公司介绍
产品分类
销售排行
Stemrd/LIF, mouse/250 µg/LIF-250
$1399.00
Stemrd/WNT-5a, mouse/25 µg/W5A-M-025
$1145.00
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